Other membranes referred to as ‘nitrocellulose’ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent. Unlike PVDF membranes, Protran nitrocellulose does not require a methanol prewetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary. High binding, low background
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions. | 
Protran BA85 Nitrocellulose Membrane |
High retention of small proteins
The 0.2 µm pore size Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through’. The 0.45 µm pore size membrane (BA85) is suitable for larger molecular weight samples. BA79, with 0.1 µm pore size, is the membrane of choice for smaller proteins below 7 kD. A key benefit of the proprietary Protran nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran. Protran blotting sandwiches
A precut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. This is made with our Protran BA83 or BA85, the highest quality NC membranes available for blotting.
For orders for NC membranes from Canada, please add a C to the end of the catalog number, for example 10485376C
Protran nitrocellulose membrane binding capacity: 80 to 150 µg/cm2; autoclavable (liquid cool cycle) |
| Ordering Information - Protran Nitrocellulose Membranes |
| Catalog Number | Description | | Protran BA83 Blotting Sandwiches |
| | 10485376 | BA83 Protran, 0.2 µm, blotting sandwich, 7 x 8.5 cm, 20/pk | | Protran BA85 Blotting Sandwiches |
| | 10485375 | BA85 Protran, 0.45 µm, blotting sandwich, 8.5 x 13.5 cm, 20/pk | | 10485374 | BA85 Protran, 0.45 µm, blotting sandwich, 7 x 8.5 cm, 20/pk | | | 10402088 | BA79 Protran, 0.1 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold 1 System, 10/pk | | 10402062 | BA79 Protran, 0.1 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk | | 10402091 | BA79 Protran, 0.1 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk | | 10484212 | BA79 Protran, 0.1 µm, blotting membrane, 33 x 56 cm, sheet, 5/pk | | 10402096 | BA79 Protran, 0.1 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk | | | 10401316 | BA83 Protran, 0.2 µm, blotting membrane, 82 mm, circle, 50/pk | | 10402426 | BA83 Protran, 0.2 µm, blotting membrane, 132 mm, circle, 50/pk | | 10402488 | BA83 Protran, 0.2 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold I System, 10/pk | | 10402493 | BA83 Protran, 0.2 µm, blotting membrane, 6.3 x 22.8 cm, sheet, fits Minifold II System, 10/pk | | 10402405 | BA83 Protran, 0.2µm, blotting membrane, 15 x 15 cm, sheet, 5/pk | | 10401465 | BA83 Protran, 0.2 µm, blotting membrane, 15 x 20 cm, sheet, 10/pk | | 10402452 | BA83 Protran, 0.2 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk | | 10401391 | BA83 Protran, 0.2 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk | | 10402453 | BA83 Protran, 0.2 µm, blotting membrane, 25 x 25 cm, sheet, 5/pk | | 10401380 | BA83 Protran, 0.2 µm, blotting membrane, 30 x 60 cm, sheet, 5/pk | | 10402480 | BA83 Protran, 0.2 µm, blotting membrane, 33 x 56 cm, sheet, 5/pk | | 10402468 | BA83 Protran, 0.2 µm, blotting membrane, 15 cm x 3 m, roll, 1/pk | | 10402495 | BA83 Protran, 0.2 µm, blotting membrane, 20 cm x 3 m, roll, 1/pk | | 10401396 | BA83 Protran, 0.2 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk | | | 10402506 | BA85 Protran, 0.45 µm, blotting membrane, 25 mm, circle, 100/pk | | 10402578 | BA85 Protran, 0.45 µm, blotting membrane, 25 mm, circle, 1000/pk | | 10401116 | BA85 Protran, 0.45 µm, blotting membrane, 82 mm, circle, 50/pk | | 10405316 | BA85 Protran, 0.45 µm, blotting membrane, 82 mm, circle with 5 mm grid, 50/pk | | 10402579 | BA85 Protran, 0.45 µm, blotting membrane, 82.5 mm, circle, 50/pk | | 10401164 | BA85 Protran, 0.45 µm, blotting membrane, 87 mm, circle, 50/pk | | 10401124 | BA85 Protran, 0.45 µm, blotting membrane, 132 mm, circle, 25/pk | | 10402525 | BA85 Protran, 0.45 µm, blotting membrane, 132 mm, circle, 50/pk | | 10401147 | BA85 Protran, 0.45 µm, blotting membrane, 137 mm, circle, 25/pk | | 10402548 | BA85 Protran, 0.45 µm, blotting membrane, 137 mm, circle, 50/pk | | 10402588 | BA85 Protran, 0.45 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold I System, 10/pk | | 10402593 | BA85 Protran, 0.45 µm, blotting membrane, 6.3 x 22.8 cm, sheet, fits Minifold II System, 10/pk | | 10401218 | BA85 Protran, 0.45 µm, blotting membrane, 8.2 x 12 cm, sheet, 10/pk | | 10402606 | BA85 Protran, 0.45 µm, blotting membrane, 15 x 15 cm, sheet, 5/pk | | 10401261 | BA85 Protran, 0.45 µm, blotting membrane, 15 x 15 cm, sheet, 25/pk | | 10402680 | BA85 Protran, 0.45 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk | | 10401191 | BA85 Protran, 0.45 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk | | 10402694 | BA85 Protran, 0.45 µm, blotting membrane, 25 x 25 cm, sheet, 5/pk | | 10401180 | BA85 Protran, 0.45 µm, blotting membrane, 30 x 60 cm, sheet, 5/pk | | 10402580 | BA85 Protran, 0.45 µm, blotting membrane, 33 x 56 cm, sheet, 5/pk | | 10402594 | BA85 Protran, 0.45 µm, blotting membrane, 15 cm x 3 m, roll, 1/pk | | 10401197 | BA85 Protran, 0.45 µm, blotting membrane, 20 cm x 3 m, roll, 1/pk | | 10401196 | BA85 Protran, 0.45 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk |
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