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Advanced Ion Exchange Celluloses

Whatman Advanced Ion Exchange Cellulose (AIEC) products are ideal for the separation of charged biopolymers and are suitable for a wide variety of applications. Available as:

ㆍPreswollen microgranular AIEC for high load capacity, fast kinetics and resolution; saves time by eliminating the need for precycling prior to buffer equilibrium
ㆍDry microgranular AIEC for similar performance characteristics as preswollen media after precycling; reduces the possibility of bacterial growth
ㆍFibrous AIEC for maximum throughput at high flow rates
ㆍCommonly used anion and cation exchange functional groups: DEAE (diethylaminoethyl tertiary amine) and CM (ether-linked carboxymethyl)
ㆍOrthophosphate bifunctional cation exchanger for sharp separation of medium molecular weight molecules

Ion Exchange Celluloses are also available in paper format.

Anion Exchange Celluloses

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작성자최고관리자 댓글 0건 조회 36회 작성일 23-09-06 11:11

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Anion Exchange Celluloses
Anion Exchange Celluloses
The DE weak (charge dependent on pH) anion exchangers are based on the diethylaminoethyl (DEAE) tertiary amine functional group. QA52 is a strong (charge independent of pH) anion exchange medium, containing quaternary amine groups.

DE23 dry fibrous DEAE cellulose
Allows fast flow rates especially after fines removal; suitable for negatively charged biopolymers.

DE32 dry microgranular DEAE cellulose
Similar performance characteristics after precycling as DE52.

DE52 preswollen microgranular DEAE cellulose
Probably the most widely used DEAE cellulose in the world; used for biopolymers with low to high negative charges; exhibits excellent resolution with good flow rates.

DE53 preswollen microgranular DEAE cellulose
Partially quaternized DEAE anion exchanger, highly substituted and with higher capacity than DE52; can be used in series with DE52 media.

QA52 preswollen microgranular QA cellulose
A strongly basic, quaternary amine-bearing anion exchange medium, moderately substituted, with high protein capacity. Fully ionized, bears constant charge under all pH conditions; excellent for high pH applications.

EXPRESS-ION High Flow Rate Ion Exchange Media are described separately.
Ion Exchange Celluloses are also available in paper format.



Typical Data - Anion Exchange Media
Phsical formFunctional groupNormal pH
range		Small ion capacity
(meq/dg*)		Protein Capacity‡Packing density
(g†/ml)
(mg/dg*)(mg/ml)
Dry Fibrous      
DE23Diethylaminoethyl2 to 9.50.88 to 1.08425600.15
Dry Microgranular     
DE32Diethylaminoethyl2 to 9.50.88 to 1.087001400.24
Preswollen Microgranular     
DE52Diethylaminoethyl2 to 9.50.88 to 1.087001300.9
DE53Diethylaminoethyl2 to 121.8 to 2.27501501.1
QA52Quaternary Ammonium2 to 121.17501501.2
* dg = dry gram;
† g = gram as supplied (dry or preswollen)
‡ Bovine serum albumin in 10 mM phosphate buffer pH 8.5


Ordering Information - Anion Exchange Celluloses
Catalog NumberDescription

Dry Fibrous DEAE Cellulose

4053-010DE23, 100 g
4053-025DE23, 250 g
Dry Microgranular DEAE Cellulose
4055-010DE32, 100 g
4055-050DE32, 500 g
Preswollen Microgranular DEAE Cellulose
4057-050DE52, 500 g
4057-200DE52, 2 kg
4058-050DE53, 500 g
4058-200DE53, 2 kg
Preswollen Microgranular Quaternary Amine Cellulose
4065-050QA52, 500 g
4065-200QA52, 2 kg
 
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